Advances in DNA sequencing technologies through Next Generation Sequencing (NGS) approaches have enabled genome-wide discovery of chromosomal copy-number variants and single-nucleotide changes. NGS technologies are rapidly expanding our ability to identify and better define disease-causing mutations and genotype-phenotype correlation. Pediatric patients may particularly benefit from the introduction of these new technologies. Pediatricians must keep up with all these new skills, both in their residency programs as well as in their continuing medical education programs.
According to current literature, sentinel lymph node identification technique is a sim-ple procedure that allows predicting the location of the axillary lymph nodes without under-staging neoplastic disease.
In clinical practice, the radio-guided surgery has been spreading also to diagnose the occult malignancy, giving space to the nuclear method for those injuries that, with a lot of difficulties for all the team's specialists, were detected using a guide-wire.
Currently the Radio-guided Occult Lesion Localization (ROLL) has become the choice procedure for all small lesions. Therefore, dedicated centers for study and treatment of breast disease use ROLL and Sentinel Node and Occult Lesion Localization (SNOLL) to resolve in a single surgery both breast disease and axillary nodal spread.
For this reason, the authors intend to prove, through this work, the idea that this method should be applied routinely in the current clinical practice of all operative units