PREVALENCE AND MOLECULAR CHARACTERIZATION OF ROTAVIRUS A IN PEDIATRIC PATIENTS WITH ACUTE DIARRHEA

Abstract

Rotaviruses are classified in the genus Rotavirus and belong to family of Reoviridae, that is

a family of viruses that can affect the gastrointestinal system. Reoviridae have genomes consisting of

segmented, double-stranded RNA (dsRNA).This study aimed to investigate the prevalence of

Rotavirus (RV) among children with acute diarrhea. One hundred and fifty pediatric patients suffering

from clinical manifestation of diarrhea, fever, and vomiting were enrolled in this study. All patients

underwent ELISA test for stool VP6 protein detection and real time PCR test for VP6 and NSP4 genes

detection. Results showed that the frequency rate of Rotavirus infection was 33.3% by ELISA

technique. The molecular techniques showed a positivity, of 33.3% for VP6 gene and 34% for NSP4

gene. The ELISA assay represented the more sensitive test in detection of Rotavirus related diarrhea in

stool specimens. The results revealed that males tend to be more effected by RV than females and the

bottle feeding children were more susceptible to virus infection, comparing to other feeding type. The

infection rate had increased with decreasing the age of the children.