Original Articles
Vol. 14 (2019)
https://doi.org/10.3269/1970-5492.2019.14.15
PREVALENCE AND MOLECULAR CHARACTERIZATION OF ROTAVIRUS A IN PEDIATRIC PATIENTS WITH ACUTE DIARRHEA
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All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
Received: October 16 2025
Published: January 12 2026
Published: January 12 2026
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Rotaviruses are classified in the genus Rotavirus and belong to family of Reoviridae, that is
a family of viruses that can affect the gastrointestinal system. Reoviridae have genomes consisting of
segmented, double-stranded RNA (dsRNA).This study aimed to investigate the prevalence of
Rotavirus (RV) among children with acute diarrhea. One hundred and fifty pediatric patients suffering
from clinical manifestation of diarrhea, fever, and vomiting were enrolled in this study. All patients
underwent ELISA test for stool VP6 protein detection and real time PCR test for VP6 and NSP4 genes
detection. Results showed that the frequency rate of Rotavirus infection was 33.3% by ELISA
technique. The molecular techniques showed a positivity, of 33.3% for VP6 gene and 34% for NSP4
gene. The ELISA assay represented the more sensitive test in detection of Rotavirus related diarrhea in
stool specimens. The results revealed that males tend to be more effected by RV than females and the
bottle feeding children were more susceptible to virus infection, comparing to other feeding type. The
infection rate had increased with decreasing the age of the children.
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How to Cite
PREVALENCE AND MOLECULAR CHARACTERIZATION OF ROTAVIRUS A IN PEDIATRIC PATIENTS WITH ACUTE DIARRHEA. (2026). EuroMediterranean Biomedical Journal, 14. https://doi.org/10.3269/1970-5492.2019.14.15
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