COLON MICROBIAL COMPOSITION IS CORRELATED WITH THE SEVERITY OF COLITIS INDUCED BY 2,4,6-TRINITROBENZENESULFONIC ACID IN MICE
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
Authors
The objective of this study was to evaluate the changes in some bacterial species of colonic microbiota, the clinical signs and the intestinal changes in mice with 2,4,6trinitobenzenesulfonic acid (TNBS)-induced colitis.
CD-1 male mice were randomly divided into three groups and inoculated intrarectally with saline, ethanol or TNBS solutions. Ethanol and TNBS treatments induced weight loss accompanied by mild and severe inflammation of the colon mucosa, respectively. However, TNBS-treated mice displayed significant differences compared to the saline group in terms of disease activity index and histological scoring. Both ethanol and TNBS groups showed an increased prevalence of Escherichia coli and Clostridium spp., decrease in Lactobacilli and Bifidobacteria counts, as well as changes in the relative proportions of bacteria in the colon.
The results confirm the validity of TNBS treatment to study the mechanisms involved in the pathogenesis and progression of inflammatory bowel diseases (IBD) in CD-1 mice. Gut microbiota may become a diagnostic biomarker with therapeutic potential for IBD in the future.
Downloads
Citations
How to Cite
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.