MULTISPECTRAL MICROSCOPY AND COMPUTERIZED IMAGE ANALYSIS: A METHOD TO OBTAIN MORE RELIABLE AND REPRODUCIBLE IMMUNOHISTOCHEMISTRY RESULTS
Abstract
The use of image analysis methods has allowed us to obtain more reliable and reproducible immunohistochemistry (IHC) results. Wider use of such approaches and simplification of software allowing a colorimetric study has meant that these methods are available to everyone, and made it possible to standardize the technique by a reliable systems score. Moreover, the recent introduction of multispectral image acquisition systems methods has further refined these techniques, minimizing artefacts and easing the evaluation of the data by the observer.
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