Dalya Shakir Al-owaidi, Moaed E. Algazally, Alaa Sadeq Alawaad
The goal of this project was to evaluate the association of candidate genetic variant rs2069502 of the CDK4 gene with breast cancer and the effect of variant genotypes on the serum concentration of CDK4 enzyme and consequently, on the occurrence of breast cancer and breast cancer subtypes in the studied population. A total of 80 breast cancer patients were divided into 4 subtypes, Luminal A, Luminal B, Her2/neu enriched and TPN, while 80 healthy individuals were enrolled as controls. Our results revealed that there were no significant differences between breast cancer patients and control groups and among breast cancer subtypes in the serum concentration of CDK4 (p-value >0.05). An allelic and genotypic association of rs2069502 with breast cancer showed that there were no significant allele frequency differences, in both alleles A and G, between breast cancer patients and the control group(p-value > 0.05). Association of rs2069502 genotypes with breast cancer and under different inheritance models revealed that there was no significant association between rs2069502 genotypes and breast cancer (p-value> 0.05). The association of rs2069502 genotypes with CDK4 serum concentration showed the presence of a significant difference between A/A and A/G, A/A and G/G in the CDK4 level (p-value < 0.05). But A/G and G/G did not show any significant difference in the CDK4 level. The rs2069502 CDK4 gene did not show any significant association with breast cancer except, in the genotyping frequency among breast cancer subtypes.