Methyldibromoglutaronitrile (MDBGN) is a preservative found in cosmetics as well as in products for industrial use. It caused an outbreak of allergic contact dermatitis in Europe in the 1990s and early 2000s. To assess the prevalence of MDBGN sensitization among consultants in the occupational dermato-allergology unit of Farhat Hached University Hospital in Sousse-Tunisia, we carried out a study of all cases of contact dermatitis to MDBGN confirmed by patch-tests from 1 January 2011 to 31 December 2015. The prevalence of allergic contact dermatitis to MDBGN was 4.5% of all cases of contact dermatitis recorded during the same period with an increase from 1.7% in 2011 to 5.4% in 2015. Associated allergens with contact dermatitis to MDBGN were the Peru balsam in 4 cases, nickel sulfate and kathon CG in 3 cases each. Contact dermatitis to Dibromodicyanobutane was associated with sensitization to other preservatives in 4 cases and cosmetic allergens in 6 cases. An increasing rates of sensitization are noticed in our region. The absence of legal restrictions regarding this preservative agent may explain an increase of its use in non-European countries.